RNA-Seq of Helianthus annuus SF342: stress R1 - SRA

SRX7646842: RNA-Seq of Helianthus annuus SF342: stress R1
1 ILLUMINA (Illumina HiSeq 2000) run: 22.3M spots, 4.5G bases, 3.3Gb downloads

Design: Leaves were cut without their petiole and immediately frozen in liquid nitrogen (greenhouse) or in dry ice (field). Grinding was performed using a ZM200 grinder (Retsch, Haan, Germany) with a 0.5-mm sieve. Total RNA was extracted using QIAzol Lysis Reagent following the manufacturers instructions (Qiagen, Dusseldorf, Germany). The RNA quality was checked by electrophoresis on an agarose gel and quality and quantity were assessed using the Agilent RNA 6000 nano kit (Agilent, Santa Clara, CA, USA). Sequencing was performed on the Illumina HiSeq 2000 by DNAVision (Charleroi, Belgium) as paired-end libraries (2*100 bp, oriented) using the TruSeq sample preparation kit (Illumina San Diego, CA, USA) according to manufacturers instructions.

Submitted by: INRAE

Study: Helianthus annuus Genome sequencing and assembly

PRJNA345532 • SRP245841 • All experiments • All runs

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Reference sequence of the sunflower inbred line XRQ/B developed by Felicity Vear at INRA Clermont Ferrand from cultivated genetic material including HA89.

Sample: SF342 FTSW 0.1

SAMN13939921 • SRS6076484 • All experiments • All runs

Organism: Helianthus annuus

Library:

Name: SF342 R1 13HP02X17

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 22.3M spots, 4.5G bases, 3.3Gb

Run	# of Spots	# of Bases	Size	Published
SRR10984322	22,274,654	4.5G	3.3Gb	2020-09-23

ID:: 9977700

SRA

Sequence Read Archive

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